{"id":25070,"date":"2019-03-13T12:00:16","date_gmt":"2019-03-13T18:00:16","guid":{"rendered":"http:\/\/www.salud.carlosslim.org\/english2\/?p=25070"},"modified":"2019-03-14T14:56:05","modified_gmt":"2019-03-14T20:56:05","slug":"edited-b-cells-produce-neutralizing-antibodies-against-hiv","status":"publish","type":"post","link":"http:\/\/www.salud.carlosslim.org\/english2\/edited-b-cells-produce-neutralizing-antibodies-against-hiv\/","title":{"rendered":"Edited B cells produce neutralizing antibodies against HIV"},"content":{"rendered":"

\"\"A researcher at the Autonomous University of Madrid (UAM) together with scientists from the Scripps Research Institute of California (USA) developed a new genome engineering technique to generate human primary B cells that produce antibodies to neutralize or disable human immunodeficiency virus (HIV)<\/strong>.<\/p>\n

The technique could be used in the future to generate new vaccines or broad-spectrum therapies, not only against HIV but against other pathogens such as influenza and hepatitis<\/strong>, the authors of the study published in eLife<\/em>.<\/p>\n

B cells<\/strong>, also called B lymphocytes<\/strong>, are responsible for producing antibodies that can recognize and attack pathogens. Although the human body can produce billions of different antibodies, those that protect against HIV are rarely generated.<\/p>\n

However there are protective antibodies against HIV. “A small percentage of patients with HIV naturally develop antibodies that can amply neutralize the virus<\/em><\/strong>,” explains Alicia Gonz\u00e1lez Mart\u00edn, co-author of the study and researcher at the UAM.<\/p>\n

“The technique we have developed edits the B-cell genome using CRISPR-Cas9 technology so that the cells produce the same neutralizing antibodies against HIV that have been found in some patients<\/em><\/strong>,” the researcher explains.<\/p>\n

B cells edited and active<\/h3>\n

The authors showed that the edited B cells still had the ability to activate, divide and go through the maturation process whereby B cells modify their own antibodies to produce more effective antibodies<\/strong>.<\/p>\n

As part of the work, the scientists also used human B cells that had been purified from the blood of three healthy donors<\/strong>. They showed once again that the edited B cells perform their normal function and produce antibodies against HIV<\/strong>.<\/p>\n

Currently there are immunotherapies against cancer in which scientists isolate patients’ T cells, genetically alter them to recognize cancer cells and return them to the bloodstream of patients to fight tumors. The new work provides the first proof of concept that a similar approach could work with B cells and infectious diseases<\/strong>.<\/p>\n

More work is needed to demonstrate that the edited B cells will work to fight pathogens in an animal model, or even in humans.<\/p>\n

“Such cells could potentially be used in preventive approaches such as a vaccine or for treatment. The next experiments will prove whether these edited cells can produce protective antibodies against HIV in response to vaccination in mice<\/em><\/strong>,” Gonz\u00e1lez Mart\u00edn concludes.<\/p>\n

 <\/p>\n

Source: <\/strong>SINC<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

A researcher at the Autonomous University of Madrid (UAM) together with scientists from the Scripps Research Institute of California (USA) developed a new genome engineering technique to generate human primary B cells that produce antibodies to neutralize or disable human immunodeficiency virus (HIV). The technique could be used in the future to generate new vaccines …<\/p>\n","protected":false},"author":7,"featured_media":25102,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"footnotes":""},"categories":[3],"tags":[11],"class_list":["post-25070","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualidad-en-la-salud","tag-vacunologia","cat-3-id","has_thumb"],"_links":{"self":[{"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/posts\/25070","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/comments?post=25070"}],"version-history":[{"count":3,"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/posts\/25070\/revisions"}],"predecessor-version":[{"id":25103,"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/posts\/25070\/revisions\/25103"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/media\/25102"}],"wp:attachment":[{"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/media?parent=25070"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/categories?post=25070"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.salud.carlosslim.org\/english2\/wp-json\/wp\/v2\/tags?post=25070"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}